part 1: Orientation of images in the MicroscopePart 2: exercise with Depth of field in the MicroscopePart 3: investigation of Pond Water & microbe

(Adapted indigenous

Parts of the Microscope:



Your microscope has 4 objective lenses: Scanning (4x), low (10x), High (40x), and also Oil Immersion (100x). In this lab, you will not usage the oil immersion lens; the is because that viewing microorganisms and requires technological instructions not spanned in this procedure.

You are watching: To focus a specimen, it is best to start with which objective

In addition to the target lenses, the ocular lens (eyepiece) has a magnification. The full magnification is identified by multiplying the magnification of the ocular and also objective lenses.

Magnification Ocular Lens Total Magnification
Scanning 4x 10x 40x
Low Power 10x 10x 100x
High Power 40x 10x 400x
Oil Immersion 100x 10x 1000x

General Procedures:

1. Make sure all backpacks, purses, etc. Are off the benchtop.

2. Carry the microscopic lense by the base and arm through both hands.

3. Keep with the cord wrapped around the microscope and the scanning objective clicked into place.

Focusing Specimens:

1. Plug your microscope right into the power supply and also switch ~ above the illuminator.

2. Always start with the stage as low as possible and making use of scanning target (4x). Odds are, friend will be able to see miscellaneous on this setting (sometimes it’s just a color). Use the coarse knob to focus: the photo may be small at this magnification, however you won"t be able to find the on the higher powers without this very first step. Move the mechanical phase until your focused image is also centered.

3. As soon as you"ve focused using the scanning objective, switch to the low strength objective (10x). Use the rough circuit knob come refocus and also move the mechanical phase to re-center your image. Again, if girlfriend haven"t focused on this level, you will not be able to move to the following level.

4. Now switch come the high power objective (40x). At this point, only use the well adjustment knob to emphasis specimens.

5. If the specimen is too light or too dark, try adjusting the diaphragm.


1. Store microscopic lense with the scanning target in place and the phase in its shortest position.

2. Wrap cords approximately the microscope.

3. Change slides to original slide tray.


Compound microscope Microscope slide with the letter “e”


Compound microscope Microscope slide v 3 threads


Compound microscopic lense Microscope on slide Coverslip carry pipette Pond water sample


1. Making use of the transport pipette, deliver a fall of pond water ~ above a microscopic lense slide. The ideal specimens commonly come from the bottom and also probably will contain chunks of birds or various other debris that you deserve to see v your naked eye.

2. Location the coverslip ~ above the slide.

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3. Use the SCANNING (4x) objective to focus, then relocate the mechanical stage around to scan the slide because that live microorganisms. You are searching for tiny swim beings- they may look eco-friendly or clear and also might be an extremely small. Pick one to emphasis on and center that in your visual field.


You may wish to usage the ProtoSlo to save your organisms from swimming too quickly!